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plasmid plvx mcherry c1  (TaKaRa)


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    Structured Review

    TaKaRa plasmid plvx mcherry c1
    Plasmid Plvx Mcherry C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+vector/pLVX-mCherry-C1+Vector/pm42880281-752-94-96
    Average 96 stars, based on 59 article reviews
    plasmid plvx mcherry c1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction.
    Article Snippet: The pLVX LifeActmScarlet plasmidwas prepared by first conducting PCR on the LifeActGFPtag2 plasmid (Ibidi USA 60101) using primers 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ and 5’-CGGTGGATCCCCTTCT TCC-3’. .. The PCR product was then cloned into the pLVX vector (Clontech #632164), which had been digested with NotI and BamHI restriction enzymes (New England Biolabs), using the In-Fusion HD enzyme kit (Takara #102518). ..

    Article Title: Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction
    Article Snippet: The pLVX LifeAct-mScarlet plasmid was prepared by first conducting PCR on the LifeAct-GFPtag2 plasmid (Ibidi USA 60101) using primers 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ and 5’-CGGTGGATCCCCTTCTTCC-3’. .. The PCR product was then cloned into the pLVX vector (Clontech #632164), which had been digested with NotI and BamHI restriction enzymes (New England Biolabs), using the In-Fusion HD enzyme kit (Takara #102518). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Clone Assay:

    Article Title: Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction.
    Article Snippet: The pLVX LifeActmScarlet plasmidwas prepared by first conducting PCR on the LifeActGFPtag2 plasmid (Ibidi USA 60101) using primers 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ and 5’-CGGTGGATCCCCTTCT TCC-3’. .. The PCR product was then cloned into the pLVX vector (Clontech #632164), which had been digested with NotI and BamHI restriction enzymes (New England Biolabs), using the In-Fusion HD enzyme kit (Takara #102518). ..

    Article Title: Cx58 is associated with the metastasis of non-small cell lung cancer via MEF2B/Cx58 axis.
    Article Snippet: All coverslips were visualized via a DMI8 confocal microscope (Leica Wetzlar, Germany). .. Lentivirus production and infection Small hairpin RNAs (shRNAs) were cloned and inserted into the pLVX vector (Takara/Clontech, Mountain View, USA). ..

    Article Title: Membrane-anchored tissue inhibitor of matrix metalloproteinase (TIMP)-1 promotes cell death in head and neck cancer by inducing DNA damage, accumulating collagen II and disrupting cell survival mechanisms.
    Article Snippet: Head and neck squamous cell carcinoma (HNSCC) remains a significant global health challenge with limited therapeutic options.. Here, we investigate a novel membrane-anchored variant of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1), designated T1Pr, as a potential therapeutic agent against HNSCC.. Utilizing a comprehensive approach involving biochemical, cellular, animal, mRNA-sequencing, and omics techniques, we demonstrate that T1Pr exhibits remarkable anti-tumorigenic effects in CAL27 cells in vitro and in vivo.

    Article Title: Cell adhesion and spreading on fluid membranes through microtubules-dependent mechanotransduction
    Article Snippet: The pLVX LifeAct-mScarlet plasmid was prepared by first conducting PCR on the LifeAct-GFPtag2 plasmid (Ibidi USA 60101) using primers 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ and 5’-CGGTGGATCCCCTTCTTCC-3’. .. The PCR product was then cloned into the pLVX vector (Clontech #632164), which had been digested with NotI and BamHI restriction enzymes (New England Biolabs), using the In-Fusion HD enzyme kit (Takara #102518). ..

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Infection:

    Article Title: Cx58 is associated with the metastasis of non-small cell lung cancer via MEF2B/Cx58 axis.
    Article Snippet: All coverslips were visualized via a DMI8 confocal microscope (Leica Wetzlar, Germany). .. Lentivirus production and infection Small hairpin RNAs (shRNAs) were cloned and inserted into the pLVX vector (Takara/Clontech, Mountain View, USA). ..

    Plasmid Preparation:

    Article Title: Cx58 is associated with the metastasis of non-small cell lung cancer via MEF2B/Cx58 axis.
    Article Snippet: All coverslips were visualized via a DMI8 confocal microscope (Leica Wetzlar, Germany). .. Lentivirus production and infection Small hairpin RNAs (shRNAs) were cloned and inserted into the pLVX vector (Takara/Clontech, Mountain View, USA). ..

    Article Title: Membrane-anchored tissue inhibitor of matrix metalloproteinase (TIMP)-1 promotes cell death in head and neck cancer by inducing DNA damage, accumulating collagen II and disrupting cell survival mechanisms.
    Article Snippet: Head and neck squamous cell carcinoma (HNSCC) remains a significant global health challenge with limited therapeutic options.. Here, we investigate a novel membrane-anchored variant of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1), designated T1Pr, as a potential therapeutic agent against HNSCC.. Utilizing a comprehensive approach involving biochemical, cellular, animal, mRNA-sequencing, and omics techniques, we demonstrate that T1Pr exhibits remarkable anti-tumorigenic effects in CAL27 cells in vitro and in vivo.

    Article Title: Targeting Hepatocellular Carcinoma Growth: Haprolid’s Inhibition of AKT Signaling Through DExH-Box Helicase 9 Downregulation
    Article Snippet: .. The pLVX-Flag-DHX9 construct was created by inserting the Flag-tagged DHX9 sequence into the pLVX vector (Clontech, San Jose, CA, USA). .. Cells were selected using 1 μg/mL puromycin (Sigma, St. Louis, MO, USA) or 500 μg/mL G418 (Sigma) for 3-7 days to isolate stably transfected cell lines.

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Construct:

    Article Title: Membrane-anchored tissue inhibitor of matrix metalloproteinase (TIMP)-1 promotes cell death in head and neck cancer by inducing DNA damage, accumulating collagen II and disrupting cell survival mechanisms.
    Article Snippet: Head and neck squamous cell carcinoma (HNSCC) remains a significant global health challenge with limited therapeutic options.. Here, we investigate a novel membrane-anchored variant of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1), designated T1Pr, as a potential therapeutic agent against HNSCC.. Utilizing a comprehensive approach involving biochemical, cellular, animal, mRNA-sequencing, and omics techniques, we demonstrate that T1Pr exhibits remarkable anti-tumorigenic effects in CAL27 cells in vitro and in vivo.

    Article Title: Targeting Hepatocellular Carcinoma Growth: Haprolid’s Inhibition of AKT Signaling Through DExH-Box Helicase 9 Downregulation
    Article Snippet: .. The pLVX-Flag-DHX9 construct was created by inserting the Flag-tagged DHX9 sequence into the pLVX vector (Clontech, San Jose, CA, USA). .. Cells were selected using 1 μg/mL puromycin (Sigma, St. Louis, MO, USA) or 500 μg/mL G418 (Sigma) for 3-7 days to isolate stably transfected cell lines.

    Sequencing:

    Article Title: Targeting Hepatocellular Carcinoma Growth: Haprolid’s Inhibition of AKT Signaling Through DExH-Box Helicase 9 Downregulation
    Article Snippet: .. The pLVX-Flag-DHX9 construct was created by inserting the Flag-tagged DHX9 sequence into the pLVX vector (Clontech, San Jose, CA, USA). .. Cells were selected using 1 μg/mL puromycin (Sigma, St. Louis, MO, USA) or 500 μg/mL G418 (Sigma) for 3-7 days to isolate stably transfected cell lines.

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Stable Transfection:

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Amplification:

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy.
    Article Snippet: .. Plasmids and stable cell line construction The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy
    Article Snippet: .. The mouse Dcn gene was PCR amplified and subcloned into the pLVX vector (632187, Clontech). ..

    Variant Assay:

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Mutagenesis:

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Binding Assay:

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..

    Cloning:

    Article Title: A universal chimeric antigen receptor (CAR)-fragment antibody binder (FAB) split system for cancer immunotherapy.
    Article Snippet: .. The sequence of protein GA1 variant, with a K33E mutation to reduce binding to human Fc (29) (TPAVTTYKLVINGRTLSGYTTTTAVDAATAEKVFKQYAYVHEVDGEWTYDDATKTFTVTEKPEKL), was cloned into a pLVX vector (Takara, 631987) via In- Fusion cloning (Takara, 638944). ..



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